Luke Shaw is one of four Saints players handed a chance to make it to the World Cup in Brazil after being named in Roy Hodgson's latest England squad.
Shaw's fellow left-backs Ashley Cole and Leighton Baines have also been included.
The game is last England international before the squad of 23 players plus seven standbys for the World Cup is selected following the end of the Premier League season.
In a boost to Lambert's chances, West Ham striker Andy Carroll has been overlooked.
James Ward-Prowse has been selected for the England under-21 side to take on Wales.
Sam Gallagher, Matt Targett, Harrison Reed and Calum Chambers are all in the under-19s squad to face Turkey next week.
Goalkeepers: Fraser Forster (Celtic), Ben Foster (West Bromwich Albion), Joe Hart (Manchester City), John Ruddy (Norwich City).
Defenders: Leighton Baines (Everton), Gary Cahill (Chelsea), Steven Caulker (Cardiff City), Ashley Cole (Chelsea), Glen Johnson (Liverpool), Luke Shaw (Southampton), Chris Smalling (Manchester United), Kyle Walker (Tottenham Hotspur).
Midfielders: Ross Barkley (Everton), Michael Carrick (Manchester United), Tom Cleverley (Manchester United), Steven Gerrard (Liverpool), Jordan Henderson (Liverpool), Adam Lallana (Southampton), Frank Lampard (Chelsea), James Milner (Manchester City), Alex Oxlade-Chamberlain (Arsenal), Raheem Sterling (Liverpool), Andros Townsend (Tottenham Hotspur), Jack Wilshere (Arsenal).
Forwards: Jermain Defoe (Toronto FC), Rickie Lambert (Southampton), Jay Rodriguez (Southampton), Wayne Rooney (Manchester United), Daniel Sturridge (Liverpool), Daniel Welbeck (Manchester United).